region-of-interest band-pass-filter statistical-significance-test metrics-evaluation diffraction-limited-spots scale-space-feature-detection total-internal-reflection-fluorescence-micscopy epifluorescence-microscopy fluorescent-speckle-microscopy low-signal-to-noise-ratio subresolution-cluster-of-fluorophores speckle-modulation-analysis imaging-technique-evaluation imaging-quality-metrics gaussian-spatial-frequencies-filtering 2d-convolution-operator image-noise-reduction bessel-function-to-gaussian-fitting vinculin latrunculin

feature-detection biological-data-analysis benchmark-framework feature-tracking live-cell-imaging ground-truth graphical-user-interface false-positive false-negative biological-data-visualization time-lapse-dataset software-benchmarking epifluorescence-microscopy benchmarking-metrics fluorescent-speckle-microscopy low-signal-to-noise-ratio software-evaluation subresolution-cluster-of-fluorophores algorithm-validation wide-field-microscopy