feature-detection biological-data-analysis benchmark-framework feature-tracking live-cell-imaging ground-truth graphical-user-interface false-positive false-negative biological-data-visualization time-lapse-dataset software-benchmarking epifluorescence-microscopy benchmarking-metrics fluorescent-speckle-microscopy low-signal-to-noise-ratio software-evaluation subresolution-cluster-of-fluorophores algorithm-validation wide-field-microscopy