calculate cis/total during balancing and use it to filter rows

[golobor]

A major practical issue with processing Hi-C data is the presence of genomic translocations. They lead errors into the calculated genomic distances and confuse cis contacts with trans ones, thus breaking the expectations of downstream analyses (obs/exp, eigenvectors).
Historically, @mimakaev and @gfudenberg dealt with these issues by filtering out genomic bins that form an untypically high fraction of trans contacts. Recently, @Phlya pointed out the need for such filters in cooler.
A simple suggestion would be to calculate the cis/total fraction in raw bins (cis_tot_raw), detect low-value outliers using MADmax and filter them out (on top of the already used MADmax-coverage filter).It may also be useful to report both the cis_tot_raw and cis_tot_balanced (i.e. cis/total fraction after filtering/balancing).

[Phlya]

Also intra-chromosomal translocations, which can, probably, be filtered similarly, but by long-range cis over total cis, or over short range cis.

[gfudenberg]

Since cis/total has a broad, locus-dependent, range influenced by mant factors, I've found that filtering on whether a bin has any extreme trans-values (e.g. equivalent to third cis diagonal) can be useful in addition to just filtering on cis/total.

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On Thu, Sep 20, 2018, 7:55 AM Ilya Flyamer ***@***.***> wrote: Also intra-chromosomal translocations, which can, probably, be filtered similarly, but by long-range cis over total cis, or over short range cis. — You are receiving this because you were mentioned. Reply to this email directly, view it on GitHub <#134 (comment)>, or mute the thread <https://github.com/notifications/unsubscribe-auth/ASvzZfQG8-WXMELGuQnETsU5fpUy7ipqks5uc6xigaJpZM4WyTaR> .

[gfudenberg]

bumping this-- @golobor suggests that cis & total sums can be set as columns during balancing

[nvictus]

See #210