Releases: nf-core/methylseq
Huggy mollusc
Pipeline Updates
- 🔄 Updated template to nf-core/tools v2.11.1 #368 @sateeshperi @edmundmiller
Bug fixes & refactoring
- 🛠 Copy methylKit-compatible files to publishDir #357 @ltosti-tagomics
- 🐛 fix ignore_r1 and ignore_3prime_r1 variable expansion #359 @noecochetel
- 🐛 No groups #362 @edmundmiller
Crema Catalana
Pipeline Updates
- 🔄 Updated template to nf-core/tools v2.9
- 🔄 Updated template to nf-core/tools v2.10
- 🔧 Updated nf-core modules for FastQC, samtools sort, samtools flagstat @ewels
- ❌ Removes problematic
-m
memory assignment for samtools sort #81
- ❌ Removes problematic
- 🧾 Use
fromSamplesheet
from nf-validation #341 @emiller88 - 🚀 Update Maintainers and add CODEOWNERS #345 @emiller88
- ⚙️ Update schema to utilize exists and add more patterns #342 @emiller88
- 📁 Support pipeline-specific configs #343 @maxulysse
Bug fixes & refactoring
- 🛠️ Added publishing of coverage (
*cov.gz
) files for NOMe-seq filtered reads forcoverage2cytosine
@FelixKrueger - 🛠️ Wrong display values for "zymo" and "em_seq" presets on help page #335 @wassimsalam01
- 📚 Use new Citation tools functions #336 @emiller88
Full Changelog: 2.4.0...2.5.0
Gillespie Gaia
Pipeline Updates
- Updated template to nf-core/tools v2.8
- Add
--bamqc_regions_file
parameter for targeted methylation sequencing data #302 - ✨ Add NF-TEST tests and snapshots for the pipeline test profile #310
Bug fixes & refactoring
- 🛠️ update index file channels to explicit value channels #310
- 🐛 fix
params.test_data_base
in test and test_full configs #310 - 🤖 GitHub Actions CI - pull_reqest to
dev
tests with NXF_VERlatest-everything
#310 - 🤖 GitHub Actions CI - pull_reqest to
master
tests with NXF_VER22.10.1
&latest-everything
#310 - 🤖 GitHub Actions CI -
fail-fast
set to false #310 - 🐛 get to the bottom of index tests #278
- ✨ Support for Bismark methylation extraction
ignore
andignore_3prime
parameters whenignore_r1
orignore_3prime_r1
are greater than 0. #322 - 🛠️ rename
ignore
->ignore_r1
andignore_3prime
->ignore_3prime_r1
params #322 - 🐛 removed unused directory #297
Pyrite Alligator
Pipeline Updates
- ⚙️ Dramatically increase the default process time config requests for Bismark and bwa meth alignment
- ✨ Add a
tower.yml
file to enable Reports in Nextflow Tower - 🤖 GitHub Actions CI - download the test data prior to running tests
Bug fixes & refactoring
- 🧹 Refactor genome indices preparation into a separate workflow
- 🧹 Refactor subworkflow logic out of alignment subworkflows, for later sharing
- 🐛 Fix a bug with using a local genome reference FASTA file
- 🐛 Fix a bunch of problems in the CI tests using nf-test (#279)
Quartzite Lemur
Pipeline Updates
- ✨ Updated the
bismark2summary
step so that it no longer stages the aligned BAM files into the working directory (#268)- Should be much faster / cheaper for running on the cloud
- ✨ Added ability to merge FastQ files based on shared IDs in sample sheet (#272)
Bug fixes & refactoring
- 🐛 Fixed typo in parameter handling for input reference indices (#263)
- 🧹 Removed orphaned
--bismark_align_cpu_per_multicore
and--bismark_align_cpu_per_multicore
parameters.- Multi-core usage for Bismark alignment is now automatically set. If you would like to overwrite this, you can do so by setting
ext.args
for the process in a custom config.
- Multi-core usage for Bismark alignment is now automatically set. If you would like to overwrite this, you can do so by setting
- 🧹 Removed duplicate option
--coverage2cytosine
(#273)- Use the existing option
--cytosine_report
to launch the newCOVERAGE2CYTOSINE
process. - Removed option
--cytosine_report genome_index
from the Bismark methylation extractor.
- Use the existing option
Granite Iguana
Pipeline Updates
- ✨ Added option to run the Bismark
coverage2cytosine
script using the--coverage2cytosine
and--nomeseq
parameters. - 🐛 Fixed bad bug where trimming presets were not being applied (#261)
Software Updates
- Update Bismark v0.23.0 to v0.24.0
Obsidian Gecko
Pipeline Updates
Major pipeline rewrite to use DSL2 with shared nf-core/modules.
Warning: Breaking change!
⚠️
The pipeline now requires a sample sheet to be passed to the pipeline with --input
:
sample | fastq_1 | fastq_2 | genome |
---|
See an example here
Note: The
genome
column is not yet used but will give the ability to map to multiple genomes in a single run in a future release. See #181.
Supplying the reference geneome with --genome
as before works as usual.
Software Updates
Major updates in commands and software versions for nearly every tool.
Please treat this new version with a little more care than usual and let us know if you find any problems!
Nauseous Serpent
Minor release to fix a bug in the command for the Bismark alignment. This change should have no effect on the pipeline output, though single-end alignments with Bismark should now be 2x faster. See issue #210 for full investigation.
Pipeline Updates
- Added new config profile to run minimal test paired-end dataset, with
-profile test_paired
. Added to the CI tests.
Bug fixes
- Fixed silent bug in Bismark alignment command that had no effect on the output (#210)
Software updates
- Picard
2.25.1
>2.25.4
- MultiQC
1.10
>1.10.1
Queasy Serpent
In line with a standardisation change across all of nf-core, we have changed the main parameter name for supplying files to the pipeline.
In this release, please use --input
instead of --reads
.
The parameter still works in the same way as before.
Pipeline Updates
- Increased resources for
fastqc
process (#143) - Raised Nextflow version requirement to
20.07.1
- Updated template to nf-core/tools 1.13.3
- Renamed
--reads
to--input
- Added new
--maxins
and--minins
parameters to pass on to Bismark - New
--em_seq
preset- Sets
bismark_maxins = 1000
,clip_r1 = 8
,clip_r2 = 8
,three_prime_clip_r1 = 8
,three_prime_clip_r2 = 8
- Sets
- New
--publish_dir_mode
parameter to customise results folder behaviour - Fix bug on AWS for
bismark_hisat
known splice file (#177) - Moved parameter documentation into new
nextflow_schema.json
file- This improves web documentation and enables
nf-core launch
functionality. See https://nf-co.re/launch?pipeline=methylseq
- This improves web documentation and enables
- Added a
-profile test_full
config for running the pipeline with a full-size test dataset- See the config file for details
- This will be used for automated release tests on AWS, results browsable on the website
Software updates
- Python base
3.7.3
>3.8.8
- markdown
3.1.1
>3.3.4
- pymdown-extensions
6.0
>8.1.1
- pygments
2.6.1
>2.8.1
- pigz
2.3.4
>2.6
- samtools
1.9
>1.11
- TrimGalore!
0.6.5
>0.6.6
- Bowtie2
2.3.5
>2.4.2
- Hisat2
2.2.0
>2.2.1
- Bismark
0.22.3
>0.23.0
- Picard
2.22.2
>2.25.1
- MethylDackel
0.5.0
>0.5.2
- MultiQC
1.8
>1.10
Belated Dodo
New features
- Added multicore support for
TrimGalore!
- Improved the multicore support for Bismark methXtract for more parallelisation (#121)
- Added
--cytosine_report
option to tell Bismark to give reports for all cytosines in the genome. - Added options
--bismark_align_cpu_per_multicore
and--bismark_align_cpu_per_multicore
to customise how Bismark align--multicore
is decided (#124)
Software updates
- New: pigz
2.3.4
- Python base
2.7
>3.7.3
- FastQC
0.11.8
>0.11.9
- TrimGalore!
0.6.4
>0.6.5
- HiSAT2
2.1.0
>2.2.0
- Bismark
0.22.2
>0.22.3
- Qualimap
2.2.2c
>2.2.2d
- Picard
2.21.3
>2.22.2
- MethylDackel
0.4.0
>0.5.0
- MultiQC
1.7
>1.8
Pipeline Updates
- Fixed bug where the iGenomes config was loaded after the references were set (#121)
- Removed awsbatch config profile because it is now served by nf-core/configs
- Tidied up the summary log messages when starting the pipeline
- Fewer messages saying what you're not doing, sanitised the order of some logs and removed a few things
- Slightly refactored the code for trimming parameters
- Updated template to tools 1.9
Bug fixes
- Fixed error where MethylDackel would consume the Nextflow channels and not work with more than one sample #140