The project (Perfomed in the Spring semester 2021) tried to determine differences between recombinant and placental calreticulin using 15N Labelling. The project is not activly maintained.
The report investigates whether the recombinant and the placental calreticulin autodigests similarly using 15N labelled recombinant calreticulin. The results from the control experiments were similar across the two types. Therefore, it is possible to use 15N labelling in such autodigestion experiments. Moreover, the autodigestion results indicate that the two calreticulin types generally autodigest similarly but some indicators of differences exist.
In addition, cysteine oxidations, which were found previously, were investigated. The results showed extensive oxidations on all cysteines and some of the methionines and prolines. The cysteine modifications indicated that calreticulin possibly could dimerise through Cys163. Moreover, the oxidations also indicated that the P-region folds down towards the core region. A possible reaction for the generation of reactive oxygen species was also proposed and involves the oxidation of water to hydrogen peroxide when the disulphide bridge is reduced to its two cysteines.
In the project the following packages where used.
For packages used in scripts, which output were used in the final report
| Name | Python Package Index | License |
|---|---|---|
| Numpy | numpy | BSD-3-Clause |
| Pandas | pandas | BSD-3-Clause |
| matplotlib | matplotlib | Python Software Foundation License 2.0 |
| matplotlib-venn | matplotlib-venn | MIT |
For packages used in scripts developed during the project, but was not used in the final report.
| Name | Python Package Index | License |
|---|---|---|
| Numpy | numpy | BSD-3-Clause |
| Pandas | pandas | BSD-3-Clause |
| matplotlib | matplotlib | Python Software Foundation License 2.0 |
| matplotlib-venn | matplotlib-venn | MIT |
| Tqdm | tqdm | Various |
| pyOpenMS | pyopenms | BSD-3-Clause |
| Pyteomics | pyteomics | Apache License 2.0 |
NB: This is NOT an endorsement of any of the abovementioned packages.
THE SOFTWARE IS PROVIDED “AS IS”, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.