Tutorials for the crisprVerse

Getting started

1. Installation
2. Building a genome index for off-target alignment
3. Specifying a custom nuclease
4. Building a gene annotation object

Common gRNA design workflows

5. CRISPR knockout (CRISPRko) design with Cas9
6. CRISPR knockout (CRISPRko) design with Cas12a
7. CRISPR knockdown (CRISPRkd) design with Cas13d
8. CRISPR base editing (CRISPRbe) design with BE4max
9. CRISPR activation (CRISPRa) design
10. CRISPR interference (CRISPRi) design

Less common gRNA design workflows

11. Paired gRNA design
12. Working with custom DNA sequences
13. Design for optical pooled screening (OPS)

Miscellaneous

14. Mapping gRNAs across species
15. Working with minor and major alleles
16. Validating existing gRNA libraries
17. Building a gRNA database across all protein-coding genes