No marker identified using run_ancombc() example

[fconstancias]

Dear @yiluheihei ,

Thanks for your efforts. Is it expected to not find any marker when running the run_ancombc() example?

data(enterotypes_arumugam)
ps <- phyloseq::subset_samples(
    enterotypes_arumugam,
    Enterotype %in% c("Enterotype 3", "Enterotype 2")
)
run_ancombc(ps, group = "Enterotype")

microbiomeMarker-class inherited from phyloseq-class
normalization method: [ none ]
microbiome marker identity method: [ ancombc ]
marker_table() Marker Table: [ no microbiome markers were identified ]
otu_table() OTU Table: [ 235 taxa and 24 samples ]
sample_data() Sample Data: [ 24 samples by 9 sample variables ]
tax_table() Taxonomy Table: [ 235 taxa by 1 taxonomic ranks ]
Warning messages:
1: The group variable has < 3 categories
The multi-group comparisons (global/pairwise/dunnet/trend) will be deactivated
2: No marker was identified

[fconstancias]

Hi @yiluheihei ,

Do you have any update on that issue? Many thanks!