Multi-variate Analysis for FLIM

Determination of Coefficient of Variation in complex FLIM datasets under different imaging conditions Main code is run through "streamlinedversion.m." Requries text file input with headers: ImageFile, KIValue, KIConcen, ManualCFDClass, FluorescentDye, Day, ROI, LaserPower, BinNumber, CollectionTime. Not all columns must contain information.

Results

• Large Cov is associated with long-lifetime FLIM data

Goals

• Compare two distinct lifetime distributions over time/ days
• Compare many distinct lifetimes over singleday/ time-lapse
• Compare live changes using a quencher
  • Translate result into biexponential dataset and biological samples
  • Examine acqusition parameters / Test pile-up correction
  • Examine solutions both hardware and software