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ngs_scripts

Repository for ngs scripts I use regularly.

List of scripts

  • align_RAD_2019.sh = alignment of RAD data from R1 and R2 fastq files (markdup, fixmate)
  • align_ind_fa_2019.sh = basic alignment of data from single fastq file
  • barcodes_pst1.csv = comma separated list of Pst1 barcodes
  • barcodes_sbf1.csv - comma separated list of Sbf1 barcodes
  • BarcodeSplitList3Files.pl = perl script to split lanes based on given barcode
  • BCsplitBestRadPE2.pl = perl script to split each lane by wells via inline barcode. NOTE right now requires barcodes to be given in line––make sure the barcodes match the RE
  • BUSCOv?.sh = script to run BUSCO version x
  • RUN_alignment_RAD.slurm = slurm script to submit for aligning RAD PE data
  • splitRAD_Pst1.sh = shell script to split lanes and barcodes of RAD data prepped with RE=Pst1
  • splitRAD_Sbf1.sh = shell script to split lanes and barcodes of RAD data prepped with RE=Sbf1

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