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Hi,
How did you set the variable features in your object? Can you try running FindVariableFeatures and set nfeatures to 479 just to extract all the features as variable features as you had previously?
This is a bug we are aware of that occurs if you run SketchData on an object in which variable features were set manually. I will push a fix shortly, but in the meantime you can try this to be able to unblock yourself.
Hi!
I am trying to run an analysis on a subset of cells from a large Xenium object:
Is there any solution to this problem?
Thanks, Chris:
Matrix products: default
BLAS/LAPACK: /fast/AG_Schmidt-Ott/tcga_data/miniconda/envs/seu5/lib/libopenblasp-r0.3.26.so; LAPACK version 3.12.0
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
time zone: Europe/Berlin
tzcode source: system (glibc)
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] Seurat_5.0.3 SeuratObject_5.0.1 sp_2.1-3 spacexr_2.2.1
loaded via a namespace (and not attached):
[1] deldir_2.0-4 pbapply_1.7-2 gridExtra_2.3
[4] rlang_1.1.3 magrittr_2.0.3 RcppAnnoy_0.0.22
[7] spatstat.geom_3.2-9 matrixStats_1.2.0 ggridges_0.5.6
[10] compiler_4.3.3 png_0.1-8 vctrs_0.6.5
[13] reshape2_1.4.4 quadprog_1.5-8 stringr_1.5.1
[16] crayon_1.5.2 pkgconfig_2.0.3 fastmap_1.1.1
[19] ellipsis_0.3.2 utf8_1.2.4 promises_1.2.1
[22] purrr_1.0.2 jsonlite_1.8.8 goftest_1.2-3
[25] later_1.3.2 spatstat.utils_3.0-4 irlba_2.3.5.1
[28] parallel_4.3.3 cluster_2.1.6 R6_2.5.1
[31] ica_1.0-3 stringi_1.8.3 RColorBrewer_1.1-3
[34] spatstat.data_3.0-4 reticulate_1.35.0 parallelly_1.37.1
[37] lmtest_0.9-40 scattermore_1.2 Rcpp_1.0.12
[40] iterators_1.0.14 tensor_1.5 future.apply_1.11.1
[43] zoo_1.8-12 sctransform_0.4.1 httpuv_1.6.14
[46] Matrix_1.6-5 splines_4.3.3 igraph_2.0.3
[49] tidyselect_1.2.1 abind_1.4-5 spatstat.random_3.2-3
[52] doParallel_1.0.17 codetools_0.2-19 miniUI_0.1.1.1
[55] spatstat.explore_3.2-7 listenv_0.9.1 lattice_0.22-6
[58] tibble_3.2.1 plyr_1.8.9 shiny_1.8.0
[61] ROCR_1.0-11 Rtsne_0.17 future_1.33.1
[64] fastDummies_1.7.3 survival_3.5-8 polyclip_1.10-6
[67] fitdistrplus_1.1-11 pillar_1.9.0 KernSmooth_2.23-22
[70] foreach_1.5.2 plotly_4.10.4 generics_0.1.3
[73] RcppHNSW_0.6.0 ggplot2_3.5.1 munsell_0.5.1
[76] scales_1.3.0 globals_0.16.3 xtable_1.8-4
[79] glue_1.7.0 lazyeval_0.2.2 tools_4.3.3
[82] data.table_1.15.4 RSpectra_0.16-1 RANN_2.6.1
[85] leiden_0.4.3.1 dotCall64_1.1-1 cowplot_1.1.3
[88] grid_4.3.3 tidyr_1.3.1 colorspace_2.1-0
[91] nlme_3.1-164 patchwork_1.2.0 cli_3.6.2
[94] spatstat.sparse_3.0-3 spam_2.10-0 fansi_1.0.6
[97] viridisLite_0.4.2 dplyr_1.1.4 uwot_0.1.16
[100] gtable_0.3.5 digest_0.6.35 progressr_0.14.0
[103] ggrepel_0.9.5 htmlwidgets_1.6.4 htmltools_0.5.8.1
[106] lifecycle_1.0.4 httr_1.4.7 mime_0.12
[109] MASS_7.3-60.0.1
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