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Thank you so much for all the work you've put into the Seurat pipeline!
I'm re-running a dataset that I ran back in March of 2024, but I'm obtaining different UMAP clustering despite using the same Seurat version (5.0.0) and the same script. Using the old annotated object I saved from my previous run as a comparison, I find that the only difference is in the scale.data of SCT which should store the Pearson residuals. Is there any reason I would get different Pearson residuals if the 'RNA' data is the same?
I checked and both objects were run with the same versions of Seurat, SCTransform, SeuratData and SeuratObjects.
Any help would be greatly appreciated. Thanks!
The text was updated successfully, but these errors were encountered:
Hi all,
Thank you so much for all the work you've put into the Seurat pipeline!
I'm re-running a dataset that I ran back in March of 2024, but I'm obtaining different UMAP clustering despite using the same Seurat version (5.0.0) and the same script. Using the old annotated object I saved from my previous run as a comparison, I find that the only difference is in the scale.data of SCT which should store the Pearson residuals. Is there any reason I would get different Pearson residuals if the 'RNA' data is the same?
I checked and both objects were run with the same versions of Seurat, SCTransform, SeuratData and SeuratObjects.
Any help would be greatly appreciated. Thanks!
The text was updated successfully, but these errors were encountered: