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Ctesi

Ctesi is a project meant to simplify processing and quantification of chemoproteomic mass spectrometry data in the Cravatt lab. The following are automated:

  • Conversion of .raw files to .ms2 and mzXML formats
  • Submission to an instance of IP2 maintained at The Scripps Research Institute
  • Quantification of data with in-house software (cimage)

Dev setup notes

Ctesi requires a copy of the cimage-minimal project, to be placed alongside it.

On development instances you must create a file config/secrets.override.yml. This will by default not be entered into version control. It must contain credentials for accessing Active Directory. There is an example file in config/secrets.yml.

You must also mount an NFS share that is accessible by a cravatt-rawprocessor (not included here) instance. An example mount commmand is included in config/mount-nfs.example.sh. This command must be issued with root privileges.