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Error executing process > 'NFCORE_NANOSEQ:NANOSEQ:QCFASTQ_NANOPLOT_FASTQC:NANOPLOT #141

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wdiao-zju opened this issue Dec 9, 2021 · 2 comments
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bug Something isn't working

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@wdiao-zju
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  It runs well in the non-basecalling, non-demultiplexing, non-alignment pipeline. However, it not runs well in the non-basecalling, non-demultiplexing pipeline. The test dataset is from nf-core/test-datasets. The version of nanoseq is v2.01. Code is as follows:

nextflow run nf-core/nanoseq
--input samplesheet_nobc_nodx.csv
--protocol cDNA
--skip_basecalling
--skip_demultiplexing
--max_cpus 40
--max_memory 100.GB
-profile docker

Error information is as follows (Note: the subsequent process such as alignment is okay, and I found only html file not png file in work/"hash dir"/fastq/MCF7_R1):
"
Error executing process > 'NFCORE_NANOSEQ:NANOSEQ:QCFASTQ_NANOPLOT_FASTQC:NANOPLOT (MCF7_R1)'

Caused by:
Missing output file(s) fastq/MCF7_R1/*.png expected by process NFCORE_NANOSEQ:NANOSEQ:QCFASTQ_NANOPLOT_FASTQC:NANOPLOT (MCF7_R1)
"

@wdiao-zju wdiao-zju added the bug Something isn't working label Dec 9, 2021
@trum994
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trum994 commented Dec 16, 2021

I'm seeing a similar issue with other nanoplot processes:

Error executing process > 'NFCORE_NANOSEQ:NANOSEQ:QCBASECALL_PYCOQC_NANOPLOT:NANOPLOT (sample1)'

Caused by:
Missing output file(s) summary/*.png expected by process NFCORE_NANOSEQ:NANOSEQ:QCBASECALL_PYCOQC_NANOPLOT:NANOPLOT (sample1)

@trum994
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trum994 commented Dec 17, 2021

@wdiao-zju if you go into that summary directory and open the *log file what's the error message that you see? I created an issue at Nanoplot here.

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