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MACS2 steps did not carried out #336

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wook2014 opened this issue Mar 5, 2023 · 2 comments
Open

MACS2 steps did not carried out #336

wook2014 opened this issue Mar 5, 2023 · 2 comments
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bug Something isn't working

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@wook2014
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wook2014 commented Mar 5, 2023

Description of the bug

I have run chipseq workflow and I can not find the MACS2 result. I have checked the log file and it seemed that the steps after deeptools did not executed.

Command used and terminal output

nextflow run ~/chipseq/nf-core-chipseq-2.0.0/workflow/ -profile singularity --input ~/chipseq/data/chipseq.csv --outdir ~/chipseq/res --fasta ~/GRCh38/GRCh38_genomic.fna --gff ~/GRCh38/GRCh38_genomic.gff --bowtie2_index ~/GRCh38/bowtie2 --gtf ~/GRCh38/GRCh38_genomic.gtf --read_length 150 --macs_gsize 2875000000 --aligner bowtie2

Relevant files

nextflow.log

System information

Nextflow version ( 22.10.6 build 5843 )
Hardware ( HPC )
Executor ( pbs )
Container engine: ( Singularity )
OS ( Linux 3.10.0-862.el7.x86_64 )
Version of nf-core/chipseq ( 2.0.0)

@wook2014 wook2014 added the bug Something isn't working label Mar 5, 2023
@lesidon
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lesidon commented May 13, 2023

I have the same issue as the author of the reported bug. wook2014, did you find the solution?

@wook2014
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I have the same issue as the author of the reported bug. wook2014, did you find the solution?

No, I run it in HPC and I don't have many permissions to debug it. I run it manually by different softwares myself later. but it still helps a lot, I don't need to set a lot of parameters in bwa and samtools to mapping.

@kittyBS kittyBS mentioned this issue May 8, 2024
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