You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
The following samples had too few reads (<1) after quality filtering with DADA2:
...
Please check whether the correct primer sequences for trimming were supplied. Ignore that samples using `--ignore_failed_filtering` or adjust the threshold with `--min_read_counts`.
but the second part is misleading, the primer sequences for trimming are here not interesting, but the truncation cutoffs, i.e. increase --trunc_qmin/--trunc_rmin or lower --trunclenf/--trunclenr, or the error filtering, i.e. increase --max_ee.
Command used and terminal output
No response
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered:
Description of the bug
The error massage says:
but the second part is misleading, the primer sequences for trimming are here not interesting, but the truncation cutoffs, i.e. increase --trunc_qmin/--trunc_rmin or lower --trunclenf/--trunclenr, or the error filtering, i.e. increase --max_ee.
Command used and terminal output
No response
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: