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Misleading error message when samples are not passing filterandtrim #733

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d4straub opened this issue Apr 11, 2024 · 0 comments
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d4straub commented Apr 11, 2024

Description of the bug

The error massage says:

The following samples had too few reads (<1) after quality filtering with DADA2:
...
Please check whether the correct primer sequences for trimming were supplied. Ignore that samples using `--ignore_failed_filtering` or adjust the threshold with `--min_read_counts`.

but the second part is misleading, the primer sequences for trimming are here not interesting, but the truncation cutoffs, i.e. increase --trunc_qmin/--trunc_rmin or lower --trunclenf/--trunclenr, or the error filtering, i.e. increase --max_ee.

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@d4straub d4straub added the bug Something isn't working label Apr 11, 2024
@d4straub d4straub added this to the 2.10.0 milestone May 7, 2024
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