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Proposal: Seqkit stats when demultiplexing #611

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a4000 opened this issue Aug 2, 2023 · 2 comments
Open

Proposal: Seqkit stats when demultiplexing #611

a4000 opened this issue Aug 2, 2023 · 2 comments
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enhancement New feature or request

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@a4000
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a4000 commented Aug 2, 2023

Description of feature

I know Ampliseq already uses fastqc, but I like using Seqkit stats when demultiplexing to track the number of reads that did and didn't get assigned to samples. There is another issue proposing a demultiplex step that I hope to resolve, so I figured I'd propose Seqkit stats for when the user chooses to demultiplex.

@a4000 a4000 added the enhancement New feature or request label Aug 2, 2023
@d4straub
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d4straub commented Aug 2, 2023

In that case I propose a demultiplexing subworkflow that contains cutadapt & seqkit to bundle the tools. I agree that it would be good to track stats for demuptiplexing, probably even a warning when a large amount of reads wasnt assigned to anything.

@a4000
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a4000 commented Aug 2, 2023

I agree on the subworklow. There are other modules I might need to add to the subworkflow to get around a problem I've identified with demultiplexing. I'll make another issue to go into more details about that problem, and my proposed solution

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