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How to solve the issule about "Found 1 bad contigs, where alphabet is less than 4 [this should not happen]" #1021

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weiguanyue opened this issue Apr 7, 2024 · 0 comments

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@weiguanyue
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when I run "funannotate predict -i testsort.fa --species "Protomyces pachydermus" -o test --cpus 25", the error is:

[Mar 25 07:14 PM]: OS: CentOS Linux 8, 104 cores, ~ 65 GB RAM. Python: 3.8.15
[Mar 25 07:14 PM]: Running funanotate v1.8.15
[Mar 25 07:14 PM]: Soft-masking simple repeats with tantan
[Mar 25 07:14 PM]: Repeat soft-masking finished:
Masked genome: /opt/wgy_m25191434_898/GCA_003717275.1mark.fa
num scaffolds: 329
assembly size: 12,007,681 bp
masked repeats: 978,989 bp (8.15%)

[Mar 25 07:14 PM]: OS: CentOS Linux 8, 104 cores, ~ 65 GB RAM. Python: 3.8.15
[Mar 25 07:14 PM]: Running funannotate v1.8.15
[Mar 25 07:14 PM]: Skipping CodingQuarry as no --rna_bam passed
[Mar 25 07:14 PM]: Parsed training data, run ab-initio gene predictors as follows:
Program Training-Method
augustus busco
genemark selftraining
glimmerhmm busco
snap busco
[Mar 25 07:14 PM]: Found 1 bad contigs, where alphabet is less than 4 [this should not happen]
[Mar 25 07:14 PM]: If you really want to keep and annotate these contigs (not recommended), pass --force

so, what should I do?

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