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Update bgd to bw conversion in callpeak_macs2.bds #5

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florianduclot
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The bedgraph to BigWig conversion step throws an error on my system for all my files:
(...)pooled.tagAlign.fc.signal.srt.bedgraph is not case-sensitive sorted at line 1879. Please use "sort -k1,1 -k2,2n" with LC_COLLATE=C, or bedSort and try again.
As mentioned in the error message and at https://gist.github.com/taoliu/2469050, using LC_COLLATE=C fixes the issue on my system when the command is ran separately (outside of chipseq.bds pipeline).

Would this addition cause conflict with the rest of the code?

Thanks,

The bedgraph to BigWig conversion step throws an error on my system for all my files:
"(...)pooled.tagAlign.fc.signal.srt.bedgraph is not case-sensitive sorted at line 1879.  Please use "sort -k1,1 -k2,2n" with LC_COLLATE=C,  or bedSort and try again."
As mentioned in the error message and at https://gist.github.com/taoliu/2469050, using "LC_COLLATE=C" fixes the issue on my system when the command is ran separately (outside of chipseq.bds pipeline).

Would this addition cause conflict with the rest of the code?

Thanks,
@leepc12
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leepc12 commented Jun 9, 2016

The funciton already has LC_COLLATE=C in line 67 of https://github.com/florianduclot/TF_chipseq_pipeline/blob/ca31df6fb4f484156eb7c098be3f938158141498/modules/callpeak_macs2.bds

But it was not exported so I modified it to export LC_COLLATE=C (in the commit 082c4a7).

@florianduclot
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Sorry I didn't notice this line before.

Thanks!

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2 participants