#SiLiCO: Simulator of Long Read Sequencing in PacBio and Oxford Nanopore
If you've downloaded the source code, unpack SiLiCO, build, and install it.
tar -xvzf SiLiCO-1.0.0.tar.gzcd SiLiCO-1.0.0python setup.py buildpython setup.py install
SiLiCO is compatible with Python 2.7.11 or higher and Python 3.5 or higher.
SiLiCO requires the following dependencies, which are installed during setup:
*pybedtools relies on the pysam package. The latest version of pysam (0.9.1.3) is problematic on OS X systems. SiLiCO installs pysam v.0.8.4, to avoid this, but installing other packages that install pysam may update it to the latest version. If you encounter a Symbol not found: _hfile_plugin_init_libcurl error when running SiLiCO, downgrade pysam with pip install pysam==0.8.4.
python SiLiCO.py -i </path/to/genome> [-o </path/to/outDir> -m <mean read length> -s <standard dev of read lengths> -c <coverage> -t <trials> -f]
[ FILE I/O ]
-i, --infile=<str>, REQ Input genome fasta file. See README for formatting requirments**.
-o, --output=<str>, OPT Output directory for results. Default = Current directory
[ DISTRIBUTION PARAMETERS ]
-m, --mean_read_length=<int>, OPT Mean read length for in-silico read generation. Default = 10000 bp
-s, --standard_dev=<int>, OPT Standard deviation of in-silico reads. Default = 2050
-c, --coverage=<int>, OPT Desired genome coverage of in-silico sequencing. Default = 8
--trials=<int>, OPT Number of trials. Default = 1
[ MODES ]
-f, --fasta, OPT FASTA Mode. When present, converts bed files to FASTA sequences using the provided reference genome.
-n, --nanopore, OPT Generate Oxford Nanopore data. Calculates a gamma distribution.
-p, --pacbio, OPT Generate PacBio data. Calculates a log normal distribution. Default mode if none specified.
[ DOCUMENTATION ]
-h, --help Display this message.
--version What version of SiLiCO are you using?
--contact Report a bug or get more help.
--citation View the citation for SiLiCO.
###Formatting Requirements
Input genome fasta should ideally be a chromosomal assembly with header lines in the style >chr1, >chr2.
If you use SiLiCO in your research please cite it as follows:
[Citation Placeholder]:
Ethan Alexander Garcia Baker, Mendivil Ramos, O., McCombie, W.R., "SiLiCO:A Simulator for Long Read Sequencing in PacBio and Oxford Nanopore". Bioinformatics. [Date]
SiLiCO is made freely available under the GNU GPL 3.0 license. This software may be freely modified and (re)distributed, but you must make your modifications freely available and cite SiLiCO.
View LICENSE.txt or http://choosealicense.com/licenses/gpl-3.0/ for more information.
(c) 2016 Ethan Alexander García Baker
