Reactive Astrocytes Omics

This project contains all the omics data used and all the scripts which were developed to do the analysis, and figure making for the Nature 2021 paper (PMID: 34616039 DOI: 10.1038/s41586-021-03960-y). Each of elovl-cko astrocytes lipidomics, reactive astrocytes lipidomics, and reactive astrocytes proteomics has a analysis.R scripts which will do the analysis for each of these sections. Separate scripts heat_maps.R and scatter_plots.R were provided to make heat maps and scatter plots figures.

All the analyzed data can be accessed through the web app: http://gliaomics.com

Raw Lipidomics Data Availability

http://massive.ucsd.edu/ProteoSAFe/status.jsp?task=ca311727ff524e32868736d3d1b3cc0a

• MassIVE MSV000087832
• Number of Files:33,959
• Total Size:1.56 GB
• Subscribers:1

Raw Proteomics Data Availability

https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=5f39b6cde74c4911951dbef1f2dac443

• MassIVE MSV000087805
• Number of Files:36
• Total Size:29.66 GB
• Subscribers:1

Requirements

• R version 4.0.2
• plyr (R library)
• tidyverse (R library)
• readxl (R library)
• ggplot2 (R library)
• ggrepel (R library)

Obtain the code

git clone https://github.com/chopralab/reactive_astrocytes_omics.git