PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.
PyPOLAR 2.6.2 improves the registration for 4 POLAR. A possibility to change the SIFT parameters (contrastThreshold, sigma) is introduced. Tuning these parameters allows for a better registration. The quality of the registration is measured by the Pearson and the Manders correlation coefficients. The values of these parameters are exported in the Excel file.
The registration is now decoupled from the choice of order for the polarization angles in 4 POLAR techniques.