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I’ve used this workflow many times and have not changed where (dirs) or how I use gemmaker but am now getting this error. I’ve deleted the .nextflow dir that is automatically made on launch in both my working dir and my home dir with no change. Online someone suggested this is happening because there is no more local storage and that is not the case for me.
Command used and terminal output
$ nextflow run systemsgenetics/gemmaker \
> -profile kamiak,singularity \
> --pipeline star \
> --input "/scidas/research/abigail.eaker/DF_assemblies/02struc_annotation/03gene/*{1,2}.fastq" \
> --star_index_dir mac-assembly.fasta.indexed \
> --star_gtf_file assembly.fasta.gtf \
> --star_keep_bamN E X T F L O W ~ version 21.10.6Launching `systemsgenetics/gemmaker` [condescending_magritte] - revision: c7fe33612e [master]------------------------------------------------------ ,--./,-. ___ __ __ __ ___ /,-._.--~' |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' systemsgenetics/gemmaker v2.1.1------------------------------------------------------Core Nextflow options revision : master runName : condescending_magritte containerEngine : singularity launchDir : /scidas/research/abigail.eaker/DF_assemblies/02struc_annotation/03gene/mac workDir : /scidas/research/abigail.eaker/DF_assemblies/02struc_annotation/03gene/mac/work projectDir : /home/abigail.eaker/.nextflow/assets/systemsgenetics/gemmaker userName : abigail.eaker profile : kamiak,singularity configFiles : /home/abigail.eaker/.nextflow/assets/systemsgenetics/gemmaker/nextflow.config, /scidas/research/abigail.eaker/DF_assemblies/02struc_annotation/03gene/mac/nextflow.configInput/output options input : /scidas/research/abigail.eaker/DF_assemblies/02struc_annotation/03gene/*{1,2}.fastq pipeline : star sras : null failed_run_report_template: /home/abigail.eaker/.nextflow/assets/systemsgenetics/gemmaker/assets/failed_sra_runs.template.html multiqc_config_file : /home/abigail.eaker/.nextflow/assets/systemsgenetics/gemmaker/assets/multiqc_config.yaml multiqc_custom_logo : /home/abigail.eaker/.nextflow/assets/systemsgenetics/gemmaker/assets/systemsgenetics-gemmaker_logo.pngKallisto Pipeline kallisto_bootstrap_samples: 0Star Pipeline star_index_dir : mac-assembly.fasta.indexed star_gtf_file : assembly.fasta.gtf star_keep_bam : trueReference genome options igenomes_ignore : trueGeneric options publish_dir_mode : link!! Only displaying parameters that differ from the pipeline defaults !!------------------------------------------------------If you use systemsgenetics/gemmaker for your analysis please cite:* The pipeline https://doi.org/10.5281/zenodo.3620945* The nf-core framework https://doi.org/10.1038/s41587-020-0439-x* Software dependencies https://github.com/systemsgenetics/gemmaker/blob/master/CITATIONS.md------------------------------------------------------[- ] process > GEMmaker:get_software_versions -[- ] process > GEMmaker:fastqc_1 -[- ] process > GEMmaker:trimmomatic -[- ] process > GEMmaker:fastqc_2 -[- ] process > GEMmaker:star -[- ] process > GEMmaker:samtools_merge -[- ] process > GEMmaker:samtools_sort -[- ] process > GEMmaker:samtools_index -[- ] process > GEMmaker:stringtie -[- ] process > GEMmaker:stringtie_fpkm_tpm -[- ] process > GEMmaker:next_sample -[- ] process > GEMmaker:multiqc -[- ] process > GEMmaker:create_gem -[- ] process > GEMmaker:clean_work_files -[- ] process > GEMmaker:clean_work_dirs -
Description of the bug
I’ve used this workflow many times and have not changed where (dirs) or how I use gemmaker but am now getting this error. I’ve deleted the .nextflow dir that is automatically made on launch in both my working dir and my home dir with no change. Online someone suggested this is happening because there is no more local storage and that is not the case for me.
Command used and terminal output
Relevant files
nextflow.log.4.zip
System information
systemsgenetics/gemmaker v2.1.1
N E X T F L O W ~ version 21.10.6
SLURM
Docker with Singularity
kamiak HPC
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