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FEATURE: <Please write a comprehensive title after the 'FEATURE: ' prefix> #680

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canfora01 opened this issue May 2, 2024 · 5 comments
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@canfora01
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Feature you'd like to see:

Hello. The electrodes we are using have the distance between each electrode extremely far apart compared to the size of the cell body. However, in kilosort clustering, several electrode-derived spikes belong to one cluster. So, is it possible to do clustering from only one electrode-derived spikes?
Thank you.

Additional Context

The version of kilosort we are using is kilosort 2.5.

@jacobpennington
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Hello @canfora01, have you tried using Kilosort4 yet? Do you encounter the same issue?

@canfora01
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Thanks for your reply.
We did try it. However, it did not work with kilosort 4 (probably because we are using a very small number of electrodes, 60 electrodes, to record from the retina, not the brain). So I did it with kilosort 2.5, which had a paper that said it recorded retinas, and it worked fine. However, the output results also showed that the response of one cell was taken from multiple electrodes. Even though there is 200µm between the electrodes.
I would be grateful if you could tell me how to solve this problem if you don't mind. Please let me know if there is anything else you would like us to try.
Thank you.

@jacobpennington
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jacobpennington commented May 4, 2024

When you say Kilosort4 "did not work," what happened exactly? Do you have some print output you can paste in here, or screenshots?

@canfora01
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Thanks for the reply. This is the error statement that was output.
IMG_2719
IMG_2720

@jacobpennington
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That looks related to some bugs that have been fixed recently. Can you please try installing the latest version and see if you still run into issues?

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