This repository contains the code used in Muscarella et al. (2019) Resource heterogeneity structures aquatic bacterial communities. ISME J
Community diversity is strongly affected by the bottom-up effects of resource availability. However, because resource pools often exist as heterogeneous mixtures of individual resources, resource heterogeneity may also affect the diversity of local communities. To test this hypothesis, we surveyed bacterial communities in lakes that spanned a resource concentration gradient. In addition, we characterized resource heterogeneity in these lakes using high-resolution mass spectrometry of the dissolved organic matter (DOM) pool (ecosystem metabolomics). Using these data, we will tested for relationships between the available resources and the aquatic heterotrophic bacteria community and used co-occurrence analysis to test for bacteria-resource interactions.
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bin: This folder contains scripts used throughout this project. These are mainly used for custom functions.
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data: This folder contains all non-sequence data needed to reproduce the analyses in this study. Some of these are output files from analyzing the raw data.
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analyses: This foler contains scripts for analyzing raw data and for producing all of the analyses used in the manuscript.
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figures: This folder contains all figures used in the manuscript and supplemental.
All sequence data is available on NCBI BioProject PRJNA540276
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Huron Mountain Wildlife Foundation Grant - Browning of freshwater ecosystems: Will terrestrial carbon loading alter the diversity and function of aquatic microbial communities?
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Indiana Academy of Sciences Grant (# 00375714) - Metabolic Fate of Terrestrial Carbon Resources
Mario Muscarella: Postdoctoral Researcher (former PhD student in Lennon Lab). Conducted the experiments and analyzed the data
Dr. Jay Lennon: Principle Investigator, Associate Professor, Department of Biology, Indiana University, Bloomington. Head of the Lennon Lab.