InfoTrim is a DNA read quality trimmer based on the Trimmomatic maximum information criterion model. It adds an entropy term. It works in both Python 2.7 and Python 3.X. It is pure Python, so there is no pip installation.
If cited, use
@inproceedings{porter2017infotrim, title={InfoTrim: A DNA read quality trimmer using entropy}, author={Porter, Jacob and Zhang, Liqing}, booktitle={Computational Advances in Bio and Medical Sciences (ICCABS), 2017 IEEE 7th International Conference on}, pages={1--2}, year={2017}, organization={IEEE} }
An extended paper can be found on the biorxiv at https://www.biorxiv.org/content/early/2017/10/11/201442
InfoTrim.py is the main program and can be run with Python. Python must be installed. InfoTrim_automate.py and the shell scripts in the shell directory were used to test the program with simulations, etc. as found in the papers. The Utilities directory contains files necessary for the program to run such as a file with constants and a generic sequence iterator (SeqIterator.py) that can iterate through FASTA and FASTQ files.
The following is the online help for the program.
Usage: InfoTrim.py [options]
Options: --version show program's version number and exit
-h, --help show this help message and exit
-p PROCESSES, --processes=PROCESSES Number of processes to use. [default: 1]
-o OUTPUT, --output=OUTPUT The file name for the output file. [default: stdout]
-s PHRED, --phred=PHRED The tradeoff value for the phred score. [default: 0.1]
-r ENTROPY, --entropy=ENTROPY The tradeoff value for the entropy feature. [default: 0.4]
-m MIN_LENGTH, --min_length=MIN_LENGTH The minimum length target. [default: 25]
-t START_WEIGHT, --start_weight=START_WEIGHT The weight for the exponential model for the starting position of the read for the non-linear option. If set to 0, it will not be used. [default: 0]
-g, --algorithm Select the algorithm. Use a linear algorithm that trims only the end. Or, a linear algorithm that trims both ends. Or, a quadratic time algorithm that trims both ends in a way that is optimal to InfoTrims scoring function.
-a ASCII_OFFSET, --ascii_offset=ASCII_OFFSET The offset value for the ascii encoding of phred scores. [default: 33]
--fasta Set this switch if the input file is a fasta file.
-b, --verbose Run in verbose. The score for each read is printed to stderr.