computer-vision quantitative-imaging cell-segmentation cell-migration active-contour image-analysis-and-processing prostate-cancer bayesian-network-inference metastasis-analysis pixel-intensity-thresholding focal-adhesion cell-cell-junction total-internal-reflection-fluorescence-microscopy oncogenic-signaling-pathways-regulation integrin-adhesion-complexes multi-parametric-analysis small-gtpase-rap1 metastasis-promoting-hormone-hgf focal-adhesion-motion-tracking focal-adhesion-morphology-analysis