Workflow of eTAM-seq data processing

Dependencies:

• Cutadapt, tested with 1.18
• Samtools, tested with 1.14
• HISAT-3N, tested with 2.2.1-3n
• pileup2var, tested with 1.1.0
• R, tested with 4.1.0. Required package: eTAMseq

eTAMseq can be downloaded from the repository and installed using the following command in R:

install.packages("eTAMseq_x.x.x.tar.gz", repos = NULL, type = "source")

Citation:

Xiao, Y., Liu S., Ge R., et al. Transcriptome-Wide Profiling and Quantification of N⁶-methyladenosine by Enzyme-Assisted Adenosine Deamination. Nat Biotechnol. 2023 Jul;41(7):9931003. PMID: 36593412