Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Seurat V5 on MERFISH data without hdf5r files #7215

Closed
YidaZhang0628 opened this issue Apr 24, 2023 Discussed in #7170 · 1 comment
Closed

Seurat V5 on MERFISH data without hdf5r files #7215

YidaZhang0628 opened this issue Apr 24, 2023 Discussed in #7170 · 1 comment

Comments

@YidaZhang0628
Copy link

Discussed in #7170

Originally posted by YidaZhang0628 April 14, 2023
I am planning to use Seurat V5 on a MERFISH dataset. The tutorial uses LoadVizgen function to read the files. However, I don't have hdf5r files from segmentation. Therefore, I won't be able to use LoadVizgen. I can read the data using ReadVizgen but it results in a plain list instead of a Seurat object. Any idea on how to read MERFISH data without segmentation information into a Seurat object?

@alikhuseynov
Copy link

alikhuseynov commented Apr 27, 2023

For Cellpose output, I submitted a PR handle Cellpose output #7162
See also this #7080
You may it devtools::install_github(repo = "alikhuseynov/seurat", ref = "feat/vizgen")
To load Vizgen this workflow could help make Seurat objects

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

3 participants