Fastq

Process fastq files

1. Demultiplex multiplexed samples prepared by TruSeq library preparation.

Usage:

perl demultiplex_Truseq.pl file.R1.fastq file.R2.fastq i1.fastq barcode.txt

a. R1 fastq file

b. R2 fastq file

c. barcode fastq file

d. Barcode file (tab separated):

  Sample1 CTTGTA
  Sample2 ATCACG