Help! Error "413 413 Request Entity Too Large"

[xpan1035]

Hello,
I am using CRISPResso2 to analyze NGS data with 31.9Mb for R2 and 29.5Mb for R1. It worked early this morning but now it keeps showing error of "413 Request Entity Too Large" even if I tried with different browsers (Firefox, chrome and edge). Is the server too busy now? Is there any suggestion I can avoid such error when using CRISPREsso2? Thank you very much!

Xiaoshu

[kclem]

Hi Xiaoshu, Does this error occur for other smaller files? We currently have an upload limit of 50M, but I just upped it to 100M so this sample should work.

Generally, for large input, it's best to run it locally via docker or conda. You can check out the installation instructions for more details.

[MsXiaoshu]

Thank you very much for replying promptly!! Really love CRISPResso! :)

[Colelyman]

Happy that CRISPResso2 is working well for you @MsXiaoshu!

[sarakn97]

Xiaoshu, I am having this exact issue. Did you fix it? I wanted to run my samples on the online application because the html output from running via conda is difficult to share via e-mail.

[sarakn97]

Hi, My R1 file is 6.7 MB and R2 is 8 MB. It was working perfectly fine earlier and now I get the "413 Request Entity Too Large" Error.
Also, Thank you so much, I will try to compress the output!
Sara

[Colelyman]

Thanks for sharing the file sizes. That definitely shouldn't be too big, I will see what we can do on our end to fix it and keep you updated!

No problem, thank you for using CRISPResso!

[Colelyman]

@sarakn97 would you mind trying again on the website? Hopefully it will work now!

[sarakn97]

I just ran it without Error on the website! Thank You!

[MsXiaoshu]

Glad to know it worked!! It worked in my case right after they increased the upload limit. But it failed again several days later when I was trying to analyze another set of data. So I switched to run it locally.

[lisahe04]

Running out the same issue right now ("413 Request Entity Too Large") and couldn't figure out how to fix it even after switching browsers. My Fastq files are about 40-60M and were ok earlier but came with this error yesterday evening. Could you please help with this? Thanks! @Colelyman