Project for the course Proteomics, applying Tandem Mass Spectrometry for protein sequencing Pipeline for tha analysis is described as following:
- Data from Tandem Mass Spectrometry are obtained and downloaded as zip file
- Extract this dataset and run python code for the whole analysis
- Python code will be excuted with:
- 3.1 Reading files from folder extracted
- 3.2 Creating database for protein and peptide
- 3.3 Creating database for b and y ions based on peptides obtained from protein database
- 3.4 Matching between the mass of tandem data and database will be counted
- 3.5 Based on the count data, the protein sequence will be listed down and ranked and scored
- Report for this pipeline analysis was written in pdf file.