Know your microscope fluorescence
PSFj analyzes a stack of images of fluorescent beads to calculate the resolution of your microscope across the fields of view. PSFj is written in JAVA and compatible with Windows, MacOS X and Linux.
Please visit PSFj website http://www.knoplab.de/psfj/
PSFj is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.