FastQc -fq|-gz either a fastq.gz|fastq
FastQc file is a binary file for linux. you can just download it and use it.
the quality bar plot meaning
5 : ----|----| ( mean of 1,2,3,4,5 position quality score )
10 : ----|----|-- ( mean of 6,7,8,9,10 position quality score )
----Nucleotide stat----
Total basepair : 1.520501861e+09
GC ratio : 38.2 %
----Reads stat----
Total reads: 5141491
Max read length: 301
Min read length: 35
Median read length: 295.73
Mean read length: 295.73
N25: 300
N50: 301
N75: 301
----Quality stat----
Mean of each postion quality score
34,34,34,34,34,37,37,37,37,37....up to end of the quality score
Median Quality score: 29.59
Mean Quality score: 29.59
N25: 23.37
N50: 32.04
N75: 36.45
Q30: 65.63 %
Q20: 72.35 %
---quality plot---
5:----|----|----|----|----|----|----
10:----|----|----|----|----|----|----|--
15:----|----|----|----|----|----|----|--
20:----|----|----|----|----|----|----|--
25:----|----|----|----|----|----|----|--
30:----|----|----|----|----|----|----|--
...
...
300:----|----|----|-