To cite this calculator, please reference the following publication:
Fisher-Wellman, K.H., Davidson, M.T., Narowski, T.M., Lin, C.-T., Koves, T.R., and Muoio, D.M. (2018). Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes. Cell Reports 24, 3593-3606.e10.
DOI: 10.1016/j.celrep.2018.08.091
PMID: 30257218
Teague, Golding, and Dobson were not involved in the generation of this site. However, their manuscript, constants, and equations formed the bedrock of this calculator.
- Teague, W. E., Golding, E. M. & Dobson, G. P. Adjustment of K’ for the creatine kinase, adenylate kinase and ATP hydrolysis equilibria to varying temperature and ionic strength. Journal of Experimental Biology 199, 509–512 (1996).
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An html rendition of the Jupyter notebook which explains the process of adjusting equilibrium constants over the creatine kinase clamp.
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A functional copy of the Jupyter notebook
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An R Shiny App to adjust and visualize the Gibbs Energy of ATP Hydrolysis (source code)
Note: To work on this code on your local computer
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LICENSE: Copyright 2017 Michael Davidson
Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM. OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.