UniFMIR

Official Implementation for "Pretraining a foundation model for generalizable fluorescence microscopy-based image restoration"

Online Demo

We provide a live demo for UniFMIR at http://unifmir.fdudml.cn/. You can also use the colab , the openxlab app or employ the following steps to run the demo locally.

User Interface for UniFMIR

1. Download the Finetuned Models

You can download the finetuned models and the examples of UniFMIR from the release. Then, you can 'tar -xzvf' the file and put the folder small model in the root directory of UniFMIR.

exampledata/
    BioSR/
    Denoise/
    Isotropic/
    Proj/
    volumetric/

experiment/
    SwinIR2t3_stage2VCD/
    SwinIRCCPs/
    SwinIRDenoising_Planaria/
    SwinIRER/
    SwinIRF-actin/
    SwinIRIsotropic_Liver/
    SwinIRMicrotubules/
    SwinIRmto1Denoising_Tribolium/
    SwinIRproj2stg_enlcn_2npzProjection_Flywing/

1. Install Packages

• Python 3.9
• Pytorch 1.10.0, CUDA 11.4 and CUDNN

pip install torch==1.10.0+cu111 torchvision==0.11.0+cu111 torchaudio==0.10.0 -f https://download.pytorch.org/whl/torch_stable.html

• Python Packages:

You can install the required python packages by the following command:

pip install -r requirements.txt

Or you can install the packages manually:

basicsr          ==          1.4.2
easydict         ==          1.10
imageio          ==          2.13.3
keras            ==          2.7.0
numpy            ==          1.21.5
opencv-python    ==          4.5.4.60
Pillow           ==          9.0.1
scikit-image     ==          0.19.2
scipy            ==          1.7.3
tensorflow-gpu   ==          2.7.0
tifffile         ==          2021.11.2
matplotlib       ==          3.5.0
protobuf         ==          3.20.3
einops           ==          0.6.0
timm             ==          0.6.11
gradio           ==          3.40.1

3. Run the Web Interface

You can run the web interface by the following command:

python app.py

Then, you can visit the web interface at http://127.0.0.1:7860/. You can upload your own image or use our examples to run UniFMIR.

Test UniFMIR

1. Prepare the datasets

All training and test data involved in the experiments are publicly available datasets. You can download our preprocessed data from the zenodo repository and unzip them into the corresponding folders. Or you can prepare the dataset according to the pretraining guidance. Then, edit the CSB_path and VCD_path in the ./small model/div2k.py file. The data path should be as follows:

• The 3D denoising/isotropic reconstruction/projection datasets:

train: CSB_path/DataSet/
[Denoising_Planaria][Denoising_Tribolium][Isotropic/Isotropic_Liver][Projection_Flywing]/train_data

test: CSB_path/DataSet/
[Denoising_Planaria][Denoising_Tribolium][Isotropic/Isotropic_Liver][Projection_Flywing]/test_data

• The SR datasets:

train: CSB_path/DataSet/BioSR_WF_to_SIM/DL-SR-main/dataset/train/[CCPs][ER][F-actin][Microtubes]

test: CSB_path/DataSet/BioSR_WF_to_SIM/DL-SR-main/dataset/test/[CCPs][ER][F-actin][Microtubes]

• The Volumetric reconstruction dataset:

train: VCD_path/vcdnet/vcd-example-data/data/train

test: VCD_path/vcdnet/vcd-example-data/data/to_predict

2. Test finetuned models

You can run the following command to test UniFMIR on given dataset or you can refer to the .py files for your adaptation. Please prepare the models according to finetuned models.

python mainSR.py # maindenoise.py # mainProjection.py # mainIsotropic.py # main2Dto3D.py

Or you can specify the precision ( single or half ) and the device (n_GPUs) by the following command:

CUDA_VISIBLE_DEVICES=0,1 python maindenoise.py --precision half --n_GPUs 2

Train UniFMIR

Please refer to the pretrain branch for training UniFMIR from scratch.

CITATION

If you use this code for your research, please cite our paper.

@article{ma2024pretraining,
  title={Pretraining a foundation model for generalizable fluorescence microscopy-based image restoration},
  author={Ma, Chenxi and Tan, Weimin and He, Ruian and Yan, Bo},
  journal={Nature Methods},
  pages={1--10},
  year={2024},
  publisher={Nature Publishing Group US New York}
}