This code quantifies the fluoresent intensity of bacteria within biofilm after the addition of a dye-encapsulated nanoemulsion to probe the interaction between the nanomaterial and the bacteria within the biofilm matrix. The time-dependent z-stacking images were taken using a Nikon A1 spectral detector confocal with FLIM module.
- Python 3
- Numpy
- Matplotlib
- Scikit-image
- nd2reader