Whole-cortex imaging in freely behaving mice using a head-mounted miniaturized microscope (mini-mScope). CMOS sensor sourced from the Miniscope.

The current version of the mini-mScope meets several experimental conditions highlighted below.

• Access to ~8x10 mm field of view of the mouse dorsal cortex
• Resolution ranges from ~39-56 micrometers across the field of view
• Weighs less than 15% of the mouse body weight (3.8 grams)
• Uniformly illuminates ~75% of the dorsal cortex with blue excitation light
• Green reflectance channel allows for hemodynamic correction ~65% of the field of view
• Does not impede mouse natural behavior (>1 hour mounting time with minimal effects on mouse traversed distance or speed)
• Posterior tail screw minimizes motion artifacts during vigorous, natural mouse behavior and during complex tasks, such as social interaction