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inconsistency in error message #229

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Qvdauwer opened this issue Nov 15, 2022 · 0 comments
Open

inconsistency in error message #229

Qvdauwer opened this issue Nov 15, 2022 · 0 comments

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@Qvdauwer
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Hello,

I would like to report a small inconsistency. I tried to run QUAST using the following code, which was wrong and my fault, because I only have one file of unpaired reads and not forward and reverse reads.

quast.py ../assemblies/Lp_M1902_trycycler.fasta -r ../../../../../../../home/qvdauwer/PhD/Genome_databases/Lbp_SK151.fna -1 ../Reads/barcode10.fastq.gz -o quast_Lp_M1902_trycycler

To which QUAST answered with this message helping me to realise my mistake.

ERROR! Use the SAME number of files with forward and reverse reads for paired-end libraries (-1 <filepath> -2 <filepath>).
Use --12 option to specify a file with interlaced forward and reverse paired-end reads.
Use -s option to specify a file with unpaired (single-end) reads.

However, as shown in the --help, -s is actually assigned to another option ( --split-scaffolds)

So this gave another error when I tried running the code again with -s instead of -1. The flag that should be used is --single.

When I changed the option to --single it worked.

This is only a small error which is easily resolved by just reading the help, but I thought you would be interested in knowing this anyway.

Best regards,
Quentin

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