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OrthoFinder Nextflow Workflows

core

flowchart TD
    p0((Channel.fromPath))
    p1[coreEntry:coreWorkflow:moveUnambiguousAminoAcidSequencesFirst]
    p2[coreEntry:coreWorkflow:orthoFinderSetup]
    p3(( ))
    p4(( ))
    p5(( ))
    p6[coreEntry:coreWorkflow:mapCachedBlasts]
    p7([splitText])
    p8([map])
    p9([toList])
    p10([splitText])
    p11([map])
    p12([toList])
    p13([map])
    p14([flatMap])
    p15([groupTuple])
    p16([collect])
    p17([collect])
    p18(( ))
    p19[coreEntry:coreWorkflow:diamond]
    p20([collect])
    p21[coreEntry:coreWorkflow:computeGroups]
    p22(( ))
    p23([flatten])
    p24([collect])
    p25([collect])
    p26([collect])
    p27(( ))
    p28[coreEntry:coreWorkflow:splitOrthologGroupsPerSpecies]
    p29([collect])
    p30[coreEntry:coreWorkflow:makeOrthogroupDiamondFiles]
    p31([flatten])
    p32([collectFile])
    p33([collect])
    p34([collect])
    p35(( ))
    p36[coreEntry:coreWorkflow:bestRepresentativesAndStats:makeFullSingletonsFile]
    p37([collate])
    p38[coreEntry:coreWorkflow:bestRepresentativesAndStats:findBestRepresentatives]
    p39([flatten])
    p40([collectFile])
    p41([concat])
    p42[coreEntry:coreWorkflow:bestRepresentativesAndStats:removeEmptyGroups]
    p43(( ))
    p44[coreEntry:coreWorkflow:bestRepresentativesAndStats:makeBestRepresentativesFasta]
    p45([splitText])
    p46[coreEntry:coreWorkflow:bestRepresentativesAndStats:filterSimilaritiesByBestRepresentative]
    p47([collect])
    p48([splitFasta])
    p49([flatten])
    p50([collate])
    p51(( ))
    p52(( ))
    p53[coreEntry:coreWorkflow:bestRepresentativesAndStats:calculateGroupResults]
    p54([collectFile])
    p55(( ))
    p56[coreEntry:coreWorkflow:bestRepresentativesAndStats:coreBestRepsToCoreDiamond]
    p57([collectFile])
    p58(( ))
    p59[coreEntry:coreWorkflow:bestRepresentativesAndStats:translateSingletonsFile]
    p60(( ))
    p61[coreEntry:coreWorkflow:bestRepresentativesAndStats:reformatGroupsFile]
    p62(( ))
    p0 -->|inputFile| p1
    p1 --> p2
    p2 --> p3
    p2 -->|setupOrthofinderWorkingDir| p16
    p2 --> p6
    p2 -->|setupSequenceMapping| p6
    p4 -->|previousDiamondCacheDirectory| p6
    p5 -->|outdatedOrganisms| p6
    p6 --> p17
    p2 --> p7
    p7 --> p8
    p8 --> p9
    p9 -->|speciesIds| p13
    p2 --> p10
    p10 --> p11
    p11 --> p12
    p12 -->|speciesNames| p23
    p13 --> p14
    p14 --> p15
    p15 -->|speciesPairsAsTuple| p19
    p16 --> p19
    p17 --> p19
    p18 -->|outputList| p19
    p19 --> p20
    p20 -->|collectedDiamondResults| p21
    p2 -->|setupOrthofinderWorkingDir| p21
    p21 -->|orthofinderGroupResultsOrthologgroups| p26
    p21 --> p22
    p23 --> p28
    p2 --> p24
    p24 --> p28
    p2 -->|setupSequenceMapping| p25
    p25 --> p28
    p26 --> p28
    p27 -->|buildVersion| p28
    p28 --> p29
    p28 -->|speciesOrthologsSingletons| p34
    p29 --> p30
    p15 -->|speciesPairsAsTuple| p30
    p20 -->|collectedDiamondResults| p30
    p30 -->|diamondSimilaritiesPerGroup| p31
    p31 --> p32
    p32 -->|allDiamondSimilaritiesPerGroup| p33
    p33 -->|allDiamondSimilarities| p46
    p34 -->|singletonFiles| p36
    p21 -->|orthofinderGroupResultsOrthologgroups| p36
    p35 -->|buildVersion| p36
    p36 --> p41
    p32 -->|allDiamondSimilaritiesPerGroup| p37
    p37 --> p38
    p38 --> p39
    p39 --> p40
    p40 --> p41
    p41 --> p42
    p42 --> p44
    p2 -->|setupOrthofinderWorkingDir| p44
    p43 -->|isResidual| p44
    p44 --> p48
    p42 --> p45
    p45 --> p46
    p36 -->|singletons| p46
    p46 --> p47
    p47 -->|groupResultsOfBestRep| p49
    p48 -->|bestRepSubset| p56
    p49 --> p50
    p50 --> p53
    p51 -->|evalueColumn| p53
    p52 -->|isResidual| p53
    p53 --> p54
    p54 --> p55
    p44 -->|bestRepsFasta| p56
    p56 --> p57
    p57 -->|bestRepsSelfDiamondResults| p58
    p36 -->|singletonsFile| p59
    p2 -->|setupSequenceMapping| p59
    p59 --> p61
    p21 -->|orthofinderGroupResultsOrthologgroups| p61
    p60 -->|buildVersion| p61
    p61 --> p62

peripheral

flowchart TD
    p0((Channel.fromPath))
    p1[peripheralEntry:peripheralWorkflow:uncompressPeripheralFastas]
    p2(( ))
    p3(( ))
    p4[peripheralEntry:peripheralWorkflow:uncompressFastas]
    p5(( ))
    p6(( ))
    p7[peripheralEntry:peripheralWorkflow:createDatabase]
    p8(( ))
    p9(( ))
    p10[peripheralEntry:peripheralWorkflow:cleanPeripheralDiamondCache]
    p11([flatten])
    p12(( ))
    p13[peripheralEntry:peripheralWorkflow:peripheralDiamond]
    p14[peripheralEntry:peripheralWorkflow:assignGroups]
    p15[peripheralEntry:peripheralWorkflow:makeResidualAndPeripheralFastas]
    p16([collectFile])
    p17([collectFile])
    p18([collectFile])
    p19[peripheralEntry:peripheralWorkflow:getPeripheralResultsToBestRep]
    p20([flatten])
    p21([collectFile])
    p22([collect])
    p23(( ))
    p24[peripheralEntry:peripheralWorkflow:combinePeripheralAndCoreSimilaritiesToBestReps]
    p25([collect])
    p26(( ))
    p27[peripheralEntry:peripheralWorkflow:combineProteomes]
    p28(( ))
    p29[peripheralEntry:peripheralWorkflow:makeGroupsFile]
    p30([splitText])
    p31(( ))
    p32[peripheralEntry:peripheralWorkflow:splitProteomeByGroup]
    p33(( ))
    p34[peripheralEntry:peripheralWorkflow:createCompressedFastaDir]
    p35(( ))
    p36[peripheralEntry:peripheralWorkflow:residualWorkflow:moveUnambiguousAminoAcidSequencesFirst]
    p37[peripheralEntry:peripheralWorkflow:residualWorkflow:orthoFinderSetup]
    p38(( ))
    p39[peripheralEntry:peripheralWorkflow:residualWorkflow:createEmptyDir]
    p40([collect])
    p41([splitText])
    p42([map])
    p43([toList])
    p44([splitText])
    p45([map])
    p46([toList])
    p47([map])
    p48([flatMap])
    p49([groupTuple])
    p50([collect])
    p51([collect])
    p52(( ))
    p53[peripheralEntry:peripheralWorkflow:residualWorkflow:diamond]
    p54([collect])
    p55[peripheralEntry:peripheralWorkflow:residualWorkflow:computeGroups]
    p56(( ))
    p57([flatten])
    p58([collect])
    p59([collect])
    p60([collect])
    p61(( ))
    p62[peripheralEntry:peripheralWorkflow:residualWorkflow:splitOrthologGroupsPerSpecies]
    p63([collect])
    p64[peripheralEntry:peripheralWorkflow:residualWorkflow:makeOrthogroupDiamondFiles]
    p65([flatten])
    p66([collectFile])
    p67([collect])
    p68([collect])
    p69(( ))
    p70[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:makeFullSingletonsFile]
    p71([collate])
    p72[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:findBestRepresentatives]
    p73([flatten])
    p74([collectFile])
    p75([concat])
    p76[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:removeEmptyGroups]
    p77(( ))
    p78[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:makeBestRepresentativesFasta]
    p79([splitText])
    p80[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:filterSimilaritiesByBestRepresentative]
    p81([collect])
    p82([splitFasta])
    p83([flatten])
    p84([collate])
    p85(( ))
    p86(( ))
    p87[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:calculateGroupResults]
    p88([collectFile])
    p89(( ))
    p90(( ))
    p91[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:mergeCoreAndResidualBestReps]
    p92[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:residualBestRepsToCoreAndResidualDiamond]
    p93([collectFile])
    p94((Channel.fromPath))
    p95([splitFasta])
    p96[peripheralEntry:peripheralWorkflow:residualWorkflow:bestRepresentativesAndStats:coreBestRepsToResidualDiamond]
    p97([collectFile])
    p98((Channel.fromPath))
    p99([concat])
    p100([concat])
    p101([collectFile])
    p102(( ))
    p0 -->|peripheralDir| p1
    p1 --> p11
    p1 --> p2
    p3 -->|inputDir| p4
    p4 --> p5
    p4 --> p27
    p6 -->|newdbfasta| p7
    p7 --> p13
    p8 -->|outdatedOrganisms| p10
    p9 -->|peripheralDiamondCache| p10
    p10 --> p13
    p11 --> p13
    p12 -->|outputList| p13
    p13 --> p14
    p13 -->|fasta| p14
    p14 --> p15
    p14 -->|diamondInput| p19
    p14 -->|fasta| p15
    p15 --> p16
    p15 --> p17
    p16 -->|residualFasta| p34
    p17 -->|peripheralFasta| p27
    p14 --> p18
    p18 -->|groupAssignments| p29
    p14 -->|groupAssignments| p19
    p19 --> p20
    p20 --> p21
    p21 --> p22
    p22 -->|peripheralSimilaritiesToBestRep| p24
    p23 -->|coreGroupSimilarities| p24
    p24 --> p25
    p25 -->|allSimilaritiesToBestRep| p26
    p27 --> p32
    p28 -->|coreGroups| p29
    p29 --> p30
    p30 --> p32
    p31 -->|outdated| p32
    p32 --> p33
    p34 -->|inputFile| p36
    p34 -->|-| p35
    p36 --> p37
    p37 --> p38
    p37 -->|setupOrthofinderWorkingDir| p50
    p37 --> p39
    p37 -->|setupSequenceMapping| p59
    p39 --> p40
    p40 -->|mappedCachedBlasts| p51
    p37 --> p41
    p41 --> p42
    p42 --> p43
    p43 -->|speciesIds| p47
    p37 --> p44
    p44 --> p45
    p45 --> p46
    p46 -->|speciesNames| p57
    p47 --> p48
    p48 --> p49
    p49 -->|speciesPairsAsTuple| p53
    p50 --> p53
    p51 --> p53
    p52 -->|outputList| p53
    p53 --> p54
    p54 -->|collectedDiamondResults| p55
    p37 -->|setupOrthofinderWorkingDir| p55
    p55 -->|orthofinderGroupResultsOrthologgroups| p60
    p55 --> p56
    p57 --> p62
    p37 --> p58
    p58 --> p62
    p59 --> p62
    p60 --> p62
    p61 -->|buildVersion| p62
    p62 --> p63
    p62 -->|speciesOrthologsSingletons| p68
    p63 --> p64
    p49 -->|speciesPairsAsTuple| p64
    p54 -->|collectedDiamondResults| p64
    p64 -->|diamondSimilaritiesPerGroup| p65
    p65 --> p66
    p66 -->|allDiamondSimilaritiesPerGroup| p67
    p67 -->|allDiamondSimilarities| p80
    p68 -->|singletonFiles| p70
    p55 -->|orthofinderGroupResultsOrthologgroups| p70
    p69 -->|buildVersion| p70
    p70 --> p75
    p66 -->|allDiamondSimilaritiesPerGroup| p71
    p71 --> p72
    p72 --> p73
    p73 --> p74
    p74 --> p75
    p75 --> p76
    p76 --> p78
    p37 -->|setupOrthofinderWorkingDir| p78
    p77 -->|isResidual| p78
    p78 --> p82
    p76 --> p79
    p79 --> p80
    p70 -->|singletons| p80
    p80 --> p81
    p81 -->|groupResultsOfBestRep| p83
    p82 -->|bestRepSubset| p92
    p83 --> p84
    p84 --> p87
    p85 -->|evalueColumn| p87
    p86 -->|isResidual| p87
    p87 --> p88
    p88 --> p89
    p78 -->|residualBestReps| p91
    p90 -->|coreBestReps.fasta| p91
    p91 --> p92
    p92 --> p93
    p93 -->|residualBestRepsSimilarities| p99
    p94 -->|coreBestRepsFasta| p95
    p95 -->|coreBestRepsFastaSubset| p96
    p78 -->|bestRepsFasta| p96
    p96 --> p97
    p97 -->|coreToResidualBestRepsSimilarities| p100
    p98 --> p99
    p99 --> p100
    p100 --> p101
    p101 --> p102

Explanation of Config File Parameters

core peripheral Parameter Value Description
X X outputDir string path Where you would like the output files to be stored
X X outdatedOrganisms string path Path to the file containing outdated organisms abbrevs (generated with checkForUpdate workflow)
X X buildVersion string Build version number
X orthoFinderDiamondOutputFields string String of outputs passed to diamond job. This should NOT be changed
X bestRepDiamondOutputFields string String of outputs passed to diamond job. This should NOT be changed
X proteomes string path Compressed directory of core organism fasta files
X diamondSimilarityCache string path Path to cache directory of diamond similarity results for the core
X coreProteomes string path Path to the input directory of core proteomes
X coreBestReps string path Path to the best representative file produced by the core workflow
X peripheralProteomes string path Path to peripheral proteomes
X coreGroupsFile string path Path to groups file output by core workflow
X peripheralDiamondCache string path Path to peripheral diamond similarity cache of peripheral to core best rep jobs
X coreSimilarityToBestReps string path Path to the group similarity results produced by the core workflow
X coreBestRepsSelfBlast string path Path to core output file indicating the which groups are similar