HT22 Genome, Epigenome, and Functional Genomics Project

Laboratory of Tychele N. Turner, Ph.D.

April 2, 2023

Project contributors:

• Tychele N. Turner, Ph.D.
• Jeffrey K. Ng
• Hillary Heins
• Juana Manuel

Project Information

In this project, we assess genomic, epigenomic, and functional genomic characteristics of the HT-22 Mouse Hippocampal Neuronal Cell Line.

Experiments we conducted include:

• Karyotype
• Illumina short-read whole-genome sequencing
• PacBio HiFi long-read whole-genome sequencing
• Illumina PolyA RNA sequencing
• PacBio Long-Read RNA Iso-Seq
• Illumina ATAC-sequencing of HT-22 cell line
• Illumina Hi-C sequencing of HT-22 cell line

Raw Data

We have deposited our raw sequencing data in SRA under our NCBI BioProject at https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA938057

Track Hub

We built a custom track hub to load in the UCSC Genome Bowser and it is available at https://data.cyverse.org/dav-anon/iplant/home/tycheleturner/Turner_Lab_Track_Hubs/HT22_genome_epigenome_functional_genomics_project/hub.txt