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updated nucleoatac documentation
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Alicia Schep committed Jun 26, 2015
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34 changes: 32 additions & 2 deletions docs/nucleoatac.md
Expand Up @@ -30,12 +30,18 @@ Outputs:

* *output_basename.occ.bedgraph.gz*: Bedgraph track with nucleosome occupancy score

* *output_basename.occ.lower_bound.bedgraph.gz*: Bedgraph track with lower bound estimate (90% confidence interval) for nucleosome occupancy score

* *output_basename.occ.upper_bound.bedgraph.gz*: Bedgraph track with upper bound estimate (90% confidence interval) nucleosome occupancy score

* *output_basename.nuc_dist.txt* and *output_basename.nuc_dist.eps*: text file and EPS plot with estimate of fragment size at nucleosomes

* *output_basename.fragmentsizes.txt*: Text file with fragment size distribution within input peaks

* *output_basename.occ_fit.txt* and *output_basename.occ_fit.eps*: text file and EPS plot of model for NFR and nucleosomal distributions



###vprocess
```
nucleoatac vprocess --sizes <sizes_file> --out <output_basename>
Expand All @@ -52,9 +58,9 @@ nucleotac nuc
```


* *output_basename.nucpos.bed*: Nucleosome dyad calls text file. Columns are: (1) chrom, (2) dyad position (0-based), (3) dyad position(1-based), (4) z-score, (5) nucleosome occupancy estimate (6) log likelihood ratio, (7) normalied nucleoatac signal value, (8) smoothed nucleoatac signal value, (9) cross-correlation signal value before normalization, (10) number of potentially nucleosome-sized fragments, (11) number of fragments smaller than nucleosome sized, (12) "fuzziness" (measure of how wide signal peak is)
* *output_basename.nucpos.bed.gz*: Nucleosome dyad calls text file. Columns are: (1) chrom, (2) dyad position (0-based), (3) dyad position(1-based), (4) z-score, (5) nucleosome occupancy estimate (6) lower bound for nucleosome occupancy estimate (90% confidence interval) 7) upper bound for nucleosome occupancy estimate (90% conficence interval) (6) log likelihood ratio, (7) normalied nucleoatac signal value, (8) smoothed nucleoatac signal value, (9) cross-correlation signal value before normalization, (10) number of potentially nucleosome-sized fragments, (11) number of fragments smaller than nucleosome sized, (12) "fuzziness" (measure of how wide signal peak is)

* *output_basename.nucpos.redundant.bed*: Includes nucleosome position calls that were within the minimum separation for non-redundant calls.
* *output_basename.nucpos.redundant.bed.gz*: Includes nucleosome position calls that were within the minimum separation for non-redundant calls.

* *output_basename.nucleoatac_signal.bedgraph.gz*: Bedgraph track with normalized cross-correlation signal

Expand All @@ -63,6 +69,30 @@ Also includes only positive signal

* *output_basename.occ.bedgraph.gz*: Bedgraph track with nucleosome occupancy score

###merge
```
nucleoatac merge
```

* *output_basename.nucmap_combined.bed.gz*: Combines low resolution nucleosome calls from occ function and higher resolution calls from nuc to create more comprehensive map


###nfr
```
nucleoatac nfr
```

* *output_basename.nfrpos.bed.gz*: NFR positions. Columns are (1) chrom, (2) left boundary (0-based), (3) right boundary (1-based), (4) mean occupancy, (5) minimum upper bound occupancy, (6) insertion density, (7) bias density













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