Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

ERR): "fastq file.fastq" does not exist. Exiting now ... #422

Open
big-boo1 opened this issue Nov 24, 2023 · 0 comments
Open

ERR): "fastq file.fastq" does not exist. Exiting now ... #422

big-boo1 opened this issue Nov 24, 2023 · 0 comments

Comments

@big-boo1
Copy link

big-boo1 commented Nov 24, 2023

Hi all,

I am running into this issue when trying to use hisat2, and was hoping someone could point me in the right direction. My code looks like

$ hisat2 -p 8 --dta -q -x SRR8846455_2.fastq -1 SRR8846456_2.fastq -s HighvsLowN1.sam

I am trying to use these 2 fastq files, and output a .sam file. However, whenever I try to run this, I get the error

(ERR): "SRR8846455_2.fastq" does not exist
Exiting now ...

I have tried adding the direct path to the files (/Users/me/fastq), copying/pasting or typing the names, running this inside and outside of my environment, and inside and outside jupyter notebook (I am using the terminal inside jupyter notebook, but it hasn't worked in the normal terminal as well).

When I enter $ ls, the files in question show up, so I know where they are. They should be recognized by my commands but I just can't get them to be used in hisat2.

Do you all have any tips?
Thank you

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant