For new animal, run MC.m & avg_fig.m accordingly. Select one averaged frames figure as template (also change the file name to m.png) and noted down coordinates of ROI for later analysis. Update Animal ID along with its ROI coordinates in process.m and you're good to go!
NOTICE: Data analysis relies on motion correction that uses codes from https://github.com/flatironinstitute/NoRMCorre and fast Tiff writing that uses codes from https://github.com/rharkes/Fast_Tiff_Write . Please follow their instructions and properly place necessary files in your folder!