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Batch effect present for some biotypes after running CombatSeq #41

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kvn95ss opened this issue Jan 26, 2024 · 0 comments
Open

Batch effect present for some biotypes after running CombatSeq #41

kvn95ss opened this issue Jan 26, 2024 · 0 comments

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@kvn95ss
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kvn95ss commented Jan 26, 2024

I'm working with smallRNA data and performed batch control with Combat_Seq (bioconductor version).

I used the below command -

library('sva')
corrected_batch <- ComBat_seq(as.matrix(counts), batch = coldata$Small_Seq_Batch)
corrected_batch <- as.data.frame(corrected_batch)

Here's a MDS plot before correcting -
MDS_before_correction

And here's the after
MDS_after_correction

What could cause this? I tried separating the biotypes first then performing the batch correction, but the outcome was pretty much the same.

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