FlowCal is a library for processing and analyzing flow cytometry data in Python. It features:
- Extraction of Flow Cytometry Standard (FCS) files into numpy array-like structures
- Traditional and non-standard gating, including automatic density-based two-dimensional gating.
- Traditional transformation functions, such as exponentiation.
- Analysis of calibration beads data, standard curve generation, and transformation to absolute units (Molecules of Equivalent Fluorophore, MEF).
- Plotting, including generation of histograms, density plots and scatter plots.
- A user-fiendly Excel UI to gate, transform, plot, and generate statistics from a list of flow cytometry samples in a simple fashion.
The official documentation can be found in https://flowcal.readthedocs.io. Here you will find installation instructions, tutorials, and more.
The official way to report a bug is through the issue tracker on github (https://github.com/taborlab/FlowCal/issues). Try to be as explicit as possible when describing your issue. Ideally, a set of instructions to reproduce the error should be provided, together with the version of all the relevant packages you are using.
Features can also be requested through the issue tracker on github. Try to be as descriptive as possible about the desired feature.