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These spikes are not part of any cluster #341

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bapungiri opened this issue Jan 25, 2021 · 9 comments
Open

These spikes are not part of any cluster #341

bapungiri opened this issue Jan 25, 2021 · 9 comments

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@bapungiri
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bapungiri commented Jan 25, 2021

I have decent number of threshold crossings (>5 std) for channels in two of the shanks. However, they are not part of any cluster.

image

Other details:

  • I initially ran the .dat file for all shanks. However, when I opened the clusters in Phy, I failed to find any clusters for these two shanks. Not sure what went wrong, I then ran the clustering for only these two shanks specifically. After running for few hours, spyking-circus crashed as it found zero templates.
  • Data length > 14 hours
  • Attached .params file
    RatK_Day2_2019-08-08_04-00-00.txt
  • Attached .prb file
    RatK_Day2_2019-08-08_04-00-00.prb.txt
  • spyking-circus version 1.0.0
@yger
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yger commented Jan 26, 2021

Please upgrade SC (1.0.6), since there might be bug fixes in between.

conda upgrade -c spyking-circus spyking-circus

Then let me know if you still have the issue

@bapungiri
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It is still failing even after upgrading spyking-circus to version 1.0.6

image

@yger
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yger commented Jan 27, 2021

From the log, it seems the data are not properly loaded. Could it be possible? What do you see when launching the preview GUI

spyking-circus mydata.dat -p

@bapungiri
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First second of filtered raw data as viewed in neuroscope:
image

Terminal message when loading the data in preview mode, nothing seems wrong here:
image

First second in the spyking-circus preview gui (I wish it was possible to preview at > 1s):
image

@yger
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yger commented Jan 27, 2021

you can

spyking-circus mydata.dat -p -s 100 (to view between 100 and 101s)

But it looks normal.. Can you send me the .log file, after the clustering?

@bapungiri
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Great, did not know that.
Actually, I ran the clustering again by changing [clustering] max_elts to 8000, which overwrote the .log file in the output folder. I can share the file after the processing is finished.

@yger
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yger commented Jan 27, 2021

What will also be good to share, in order to debug/understand what is happening, is to set make_plots = png in the [clustering] section. And send me the zipped plot folder in the results

@bapungiri
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Sure will do.

@bapungiri
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Attached .log and .png files
outputfiles.zip

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