diff --git a/snakemake/deployment/conda.py b/snakemake/deployment/conda.py
index 59a91a556..8bb7327b9 100644
--- a/snakemake/deployment/conda.py
+++ b/snakemake/deployment/conda.py
@@ -62,8 +62,14 @@ def __init__(
     ):
         self.file = None
         self.name = None
+        self.post_deploy_file = None
         if env_file is not None:
             self.file = infer_source_file(env_file)
+            deploy_file = Path(self.file.get_path_or_uri()).with_suffix(
+                ".post-deploy.sh"
+            )
+            if deploy_file.exists():
+                self.post_deploy_file = infer_source_file(deploy_file)
         if env_name is not None:
             assert env_file is None, "bug: both env_file and env_name specified"
             self.name = env_name
@@ -101,8 +107,9 @@ def _get_content(self):
 
     def _get_content_deploy(self):
         self.check_is_file_based()
-        deploy_file = Path(self.file).with_suffix(".post-deploy.sh")
-        return self.workflow.sourcecache.open(deploy_file, "rb").read()
+        if self.post_deploy_file:
+            return self.workflow.sourcecache.open(self.post_deploy_file, "rb").read()
+        return None
 
     @property
     def _env_archive_dir(self):
@@ -139,6 +146,9 @@ def hash(self):
                 md5hash.update(env_dir.encode())
                 if self._container_img:
                     md5hash.update(self._container_img.url.encode())
+                content_deploy = self.content_deploy
+                if content_deploy:
+                    md5hash.update(content_deploy)
                 md5hash.update(self.content)
                 self._hash = md5hash.hexdigest()
         return self._hash
@@ -148,6 +158,9 @@ def content_hash(self):
         if self._content_hash is None:
             md5hash = hashlib.md5()
             md5hash.update(self.content)
+            content_deploy = self.content_deploy
+            if content_deploy:
+                md5hash.update(content_deploy)
             self._content_hash = md5hash.hexdigest()
         return self._content_hash
 
@@ -317,11 +330,7 @@ def create(self, dryrun=False):
                 tmp.write(self.content)
                 env_file = tmp.name
                 tmp_env_file = tmp.name
-            if (
-                Path(self.file.get_path_or_uri())
-                .with_suffix(".post-deploy.sh")
-                .exists()
-            ):
+            if self.post_deploy_file:
                 with tempfile.NamedTemporaryFile(
                     delete=False, suffix=".post-deploy.sh"
                 ) as tmp:
@@ -331,8 +340,7 @@ def create(self, dryrun=False):
                     tmp_deploy_file = tmp.name
         else:
             env_file = env_file.get_path_or_uri()
-            if Path(env_file).with_suffix(".post-deploy.sh").exists():
-                deploy_file = Path(env_file).with_suffix(".post-deploy.sh")
+            deploy_file = self.post_deploy_file
 
         env_path = self.address
 
diff --git a/tests/test_deploy_hashing/Snakefile b/tests/test_deploy_hashing/Snakefile
new file mode 100644
index 000000000..9fe47395a
--- /dev/null
+++ b/tests/test_deploy_hashing/Snakefile
@@ -0,0 +1,19 @@
+rule all:
+    input:
+        expand("{s}.txt", s=["a", "b"])
+
+rule a:
+    output:
+        "a.txt"
+    conda:
+        "a.yaml"
+    shell:
+        "touch {output}"
+
+rule b:
+    output:
+        "b.txt"
+    conda:
+        "b.yaml"
+    shell:
+        "touch {output}"
\ No newline at end of file
diff --git a/tests/test_deploy_hashing/a.post-deploy.sh b/tests/test_deploy_hashing/a.post-deploy.sh
new file mode 100644
index 000000000..ebca5bab2
--- /dev/null
+++ b/tests/test_deploy_hashing/a.post-deploy.sh
@@ -0,0 +1,3 @@
+#!/bin/bash
+
+echo "test" > $CONDA_PREFIX/a.txt
\ No newline at end of file
diff --git a/tests/test_deploy_hashing/a.yaml b/tests/test_deploy_hashing/a.yaml
new file mode 100644
index 000000000..50d99e291
--- /dev/null
+++ b/tests/test_deploy_hashing/a.yaml
@@ -0,0 +1,5 @@
+channels:
+  - bioconda
+  - conda-forge
+dependencies:
+  - python =3.10
diff --git a/tests/test_deploy_hashing/b.yaml b/tests/test_deploy_hashing/b.yaml
new file mode 100644
index 000000000..50d99e291
--- /dev/null
+++ b/tests/test_deploy_hashing/b.yaml
@@ -0,0 +1,5 @@
+channels:
+  - bioconda
+  - conda-forge
+dependencies:
+  - python =3.10
diff --git a/tests/test_deploy_hashing/expected-results/a.txt b/tests/test_deploy_hashing/expected-results/a.txt
new file mode 100644
index 000000000..e69de29bb
diff --git a/tests/test_deploy_hashing/expected-results/b.txt b/tests/test_deploy_hashing/expected-results/b.txt
new file mode 100644
index 000000000..e69de29bb
diff --git a/tests/tests.py b/tests/tests.py
index 2e54c4c2f..293c55747 100644
--- a/tests/tests.py
+++ b/tests/tests.py
@@ -434,6 +434,12 @@ def test_deploy_script():
     run(dpath("test_deploy_script"), use_conda=True)
 
 
+@skip_on_windows
+def test_deploy_hashing():
+    tmpdir = run(dpath("test_deploy_hashing"), use_conda=True, cleanup=False)
+    assert len(next(os.walk(os.path.join(tmpdir, ".snakemake/conda")))[1]) == 2
+
+
 def test_conda_custom_prefix():
     run(
         dpath("test_conda_custom_prefix"),