diff --git a/snakemake/workflow.py b/snakemake/workflow.py
index 8354c44a4..74d10508e 100644
--- a/snakemake/workflow.py
+++ b/snakemake/workflow.py
@@ -1484,6 +1484,9 @@ def decorate(ruleinfo):
                         rule=rule,
                     )
 
+                if isinstance(ruleinfo.conda_env, Path):
+                    ruleinfo.conda_env = str(ruleinfo.conda_env)
+
                 if (
                     ruleinfo.conda_env is not None
                     and is_conda_env_file(ruleinfo.conda_env)
diff --git a/tests/test_conda/Snakefile b/tests/test_conda/Snakefile
index 4f449adb3..06214a211 100644
--- a/tests/test_conda/Snakefile
+++ b/tests/test_conda/Snakefile
@@ -1,4 +1,5 @@
 shell.executable("bash")
+conda_env = Path("test-env.yaml")
 
 rule all:
     input:
@@ -19,6 +20,6 @@ rule b:
     output:
         "test{i}.out2"
     conda:
-        "test-env.yaml"
+        conda_env
     shell:
         "Tm -h > {output} || true"