estimate TMB in tumor only variants

[yihancc]

Hi! Thanks for this wonderful tool!

I used varlociraptor for tumor only variant calling, and now I want to use its "estimate mutational-burden" funtion to estimate the TMB in my data. I tried this codevarlociraptor estimate mutational-burden --coding-genome-size 6.5e3 --events SOMATIC_TUMOR+GERMILNE_TUMOR --plot-mode curve --sample calls_anno.vcf, but I got nothing even the error message.

Can I use "estimate mutational-burden" to estimate TMB in the tumor only variants? If so, is there anything wrong with my usage?

Thank you for your time!