Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Crosslink user controlled protein N-terminal as a modification site #2312

Open
lonelu opened this issue Sep 19, 2023 · 1 comment
Open

Crosslink user controlled protein N-terminal as a modification site #2312

lonelu opened this issue Sep 19, 2023 · 1 comment
Assignees

Comments

@lonelu
Copy link
Member

lonelu commented Sep 19, 2023

So far, protein Nter is included as a modification site. In theory, it should be, as Nter ammonium group (NH3+) is a good reactive spot.
The function related is here:
MetaMorpheus/EngineLayer/CrosslinkSearch
/CrosslinkSpectralMatch.cs. -> GetPossibleCrosslinkerModSites

@lonelu lonelu self-assigned this Sep 19, 2023
@ACE-Manager
Copy link

Hi Lei(?)...
thanks for taking care of my issue. Just to make clear what my problem is ... In other CL programs like MeroX you have to specifically add the N-term as a site. This is done when you setup the cross linker. You have to add a wavy bracket ({= N-term; }= Cterm). In MM I do not know how to set this up. I do not know what the respective symbol is. Or is the N-term search done automatically, anyway?

With regard to the path your answer (MetaMorpheus/EngineLayer/CrosslinkSearch
/CrosslinkSpectralMatch.cs. -> GetPossibleCrosslinkerModSites) ... where do I find this? My MM installation does not have this path ... I am using MM 1.0.3.

Thanks for your help.
Sincerely yours
Farnusch

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants